Optofluidics is dedicated to achieving integrated control of particle and fluid motion, particularly on the micrometer scale, by utilizing light to direct fluid flow and particle motion. The field has seen significant growth recently, driven by the concerted efforts of researchers across various scientific disciplines, notably for its successful applications in biomedical science. In this review, we explore a range of optofluidic architectures developed over the past decade, with a primary focus on mechanisms for precise control of micro and nanoscale biological objects and their applications in sensing. Regarding nanoparticle manipulation, we delve into mechanisms based on optical nanotweezers using nanolocalized light fields and light-based hybrid effects with dramatically improved performance and capabilities. In the context of sensing, we emphasize those works that used optofluidics to aggregate molecules or particles to promote sensing and detection. Additionally, we highlight emerging research directions, encompassing both fundamental principles and practical applications in the field.